In order to investigate the cytotoxicity of composite resin in vitro, BALB/C mouse fibroblast were cultured in MEM in which silux, P-50, microrest, clearfil, amalgam and glass-ionomer, in shape of 2×9㎜ circular disk.
The experiments were performed by cell count on 4 hours, 1, 3, 6 days and the composite resin groups, amalgam, glass-ionomer were compared.
1. On the sixth day, the cellular number of resin composite groups were remarkedly reduced, in contrast, the that of amalgam and glass-ionomer group continuously increased.
2. It was only on the 4 hours that the cellular number contained in amalgam were reduced, but increased thereafter, and the cellular number contained in glass-ionomer are greater than other groups.
3. In resin group, especially between self-curing resin and light-curing resin, there is no difference in cellular number statistically(p>0.05).
4. It was amalgam where the round cell without cellular process was found on the 4 hours and on the 6th day the cell without cellular process was found numeroulsy in resin group whereas in amalgam and glass-ionomer, like control group was contained cell forming monolayer.
These result suggested that the toxicity of the self-curing and light-curing resin greater than that of the amalgam and galss-ionomer.
|